Plasmid

Part:BBa_K3482026:Design

Designed by: Thierry Marti   Group: iGEM20_UNILausanne   (2020-10-23)


pLPT41 sponge plasmid(Potvin-Trottier, 2016)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 87
    Illegal EcoRI site found at 172
    Illegal SpeI site found at 1133
    Illegal PstI site found at 121
    Illegal PstI site found at 178
    Illegal PstI site found at 1882
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 87
    Illegal EcoRI site found at 172
    Illegal SpeI site found at 1133
    Illegal PstI site found at 121
    Illegal PstI site found at 178
    Illegal PstI site found at 1882
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 87
    Illegal EcoRI site found at 172
    Illegal BglII site found at 2095
    Illegal BamHI site found at 190
    Illegal XhoI site found at 7
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 87
    Illegal EcoRI site found at 172
    Illegal SpeI site found at 1133
    Illegal PstI site found at 121
    Illegal PstI site found at 178
    Illegal PstI site found at 1882
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 87
    Illegal EcoRI site found at 172
    Illegal SpeI site found at 1133
    Illegal PstI site found at 121
    Illegal PstI site found at 178
    Illegal PstI site found at 1882
    Illegal NgoMIV site found at 1431
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1371
    Illegal SapI site found at 1581


Design Notes

Temporary


Source

This plasmid originates from following publication: doi: 10.1038/nature19841.

References